Biologically active substances of topping for people with static and physical activity

Abstract

Background. Qualitative and quantitative content of the finished product of biologically active substances is the main criterion for selecting toppings for people with static and physical activity, because their positive impact on the functional state of the organism determines the expediency of product development and research of its effectiveness.
The aim of the research is to develop a product for people with long-term static and physical activity, which would prevent the development of diseases of the joints and fatigue, with the inclusion of biologically active compounds L-carnitine and glucosamine in the formulation of topping.
Material and methods. As biologically active substances L-carnitine and glucosamine where used in the topping, their identification was carried out by high performance liquid chromatography method. This method is convenient because of the availability to use preparative isolation and to conduct qualitative and quantitative analysis of non-volatile thermally labile compounds with both small and high molecular weight.
Results. According to the experimental results 1 g of toppings contains 20 mg / g of glucosamine (at a rate add to the formulation 18–22 mg / g) and 16 mg / g of L-carnitine (at a rate of 14.4–17.6 mg / g).
Conclusion. By using HPLC the identification of biologically active substances toppings was carried out, including L-carnitine and glucosamine. Their quantitative content in the product was established.